Volume 24, Issue 101, January - February, 2020

Seroprevalence and 16S rRNA gene sequence analysis of Brucella spp. among domestic ruminants in Northern Border, Saudi Arabia

Muaz Magzob Abdellatif^1,2♦, Yahia Hassan Ali Osman^1,3, Hussam Hassan Arafat^1,4, Ahmed Zein Elabdeen Mahmoud⁵

¹Department of Biology, Faculty of Science and Arts, Northern Border University, Rafha, KSA
²Department of Microbiology, Faculty of Vet. Science. University of Nyala, Sudan
³Department of Virology, Central Veterinary Research Laboratory, Khartoum, Sudan
⁴Department of Botany &Microbiology, Faculty of Science, Minia University, Minia City- 61519, Egypt
⁵Veterinary Laboratories, Ministry of Environment, Water and Agriculture, Saudi Arabia

^♦Corresponding author
Muaz Magzob Abdellatif, Northern Border University, Saudi Arabia, Email: muaz.mohamed@nbu.edu.sa

ABSTRACT

Brucellosis is a worldwide bacterial zoonotic disease. In this study, seroprevalence of Brucella spp. among domestic ruminants in Hail, Saudi Arabia was investigated and strains recovered from camels were identified based on 16S rRNA gene sequence analysis. The overall seropositivity was 10.6%, goats had a higher seroprevalence of 12.1% compared to 11.4% in sheep and 6.2% for camels as tested by RBPT. Nucleotide identity of 16S rRNA among Hail camel brucella, strains was 99.9%, while identity with Brucella strains recovered from GenBank ranged from 100 to 89.3%. To put an efficient control program in action, further research to identify the strains involved; highlights potential risks that maintain the infection in animals and/or the environment should be done.

Keywords: Seroprevalence, Brucella, 16S rRNA gene, Domestic Ruminants, Hail, Saudi Arabia

Medical Science, 2020, 24(101), 165-173

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