Volume 26, Issue 126, August 2022

Laser polarimetry of human biological tissues as an effective method of differential diagnosis, using the prostate gland as an example

Vyacheslav Sokol

Department of forensic medicine, medical law named after Professor M.S. Bocarius, Kharkiv National Medical University, Kharkiv, Ukraine

ABSTRACT

Laser polarimetric technologies (LPT) for the assessment of biological tissues (BT) are a promising scientific direction of research, which is based on the evaluation of changes in the light beam that illuminates the examined tissue. These methods have proven themselves well in many medical fields, such as oncology, gynecology, operative surgery, pathological anatomy, forensic medicine, etc., and allow us to establish pathological changes in BT, the temporal dynamics of these changes, and also expand our knowledge about the morphological structure of various tissues and body systems. In this work, we demonstrated the possibility of using Muller-matrix polarization tomography (MMPT) of linear birefringence (LB) of fibrillar structures of histological preparations of the prostate gland (PG) for the recognition of adenomas, poorly differentiated carcinomas and normal tissues. Histological sections of PG (normal n=30, adenoma n=60, carcinoma n=60) were studied by means of differential MMPT LB of fibrillar structures of preparations with further statistical processing of the obtained results. Calculations of the M, V, As and Ex, which characterize the polarization-reproduced diffuse tomograms of the LB were statistically significant (p1; p2; p1;2<0.05) for diagnostic use in differentiation tumor processes of the PG. As a result of the calculation of the balanced accuracy of statistical processing of coordinate distributions of the LB value of all groups for the differential diagnosis of adenoma and poorly differentiated prostate carcinoma samples in comparison with normal tissue, satisfactory (V→81.3%) and excellent level of values (M, As, Ex →85-93%) were established).

Keywords: biological tissues, polarization, laser polarimetry, laser, microscopic samples, Mueller's matrix

Medical Science, 2022, 26, ms337e2430

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DOI: https://doi.org/10.54905/disssi/v26i126/ms337e2430

Published: 11 August 2022