Volume 25, Issue 118, December 2021

Time-dependent expression patterns of inflammatory markers in rat model of lipopolysaccharide-induced acute systemic inflammation

Eman Mohammad Albataineh¹, Saad Abdulrahman Hussain², Sinan Subhi Farhan³, Heba M Abd El Kareem⁴, Samir Saad Mahgoub^5♦

¹Department of Microbiology and Pathology, Faculty of Medicine, Mutah University, Al-Karak, Jordan
²Department of Pharmacology and Toxicology, Faculty of Pharmacy, AlRafidain University College, Baghdad, Iraq
³Department of Basic Sciences, Faculty of Pharmacy, Al-Rafidain University College, Baghdad, Iraq
⁴Department of Biochemistry and Molecular Biology Faculty of Medicine, Mutah University, Jordan, Department of Biochemistry and Molecular Biology, Faculty of Medicine, Benha University, Egypt
⁵Department of Biochemistry and Molecular Biology Faculty of Medicine, Mutah University, Jordan, Department of Biochemistry and Molecular Biology, Faculty of Medicine, Al-Minia University, Egypt

^♦Corresponding author
Department of Biochemistry and Molecular Biology Faculty of Medicine, Mutah University, Jordan, Department of Biochemistry and Molecular Biology, Faculty of Medicine, Al-Minia University, Egypt

ABSTRACT

Objectives: The study aimed to assess the relationship between various inflammatory markers in an induced systemic inflammation by lipopolysaccharide (LPS) after 6 and 24-hr intervals, and to compare their sensitivity and specificity. Materials and methods: Two hundred and seventy rats were allocated into two groups; 150 rats (control group) and 120 rats (test group). The control group was injected i.p with saline, while the test group was injected with 100 μg/kg of LPS i.p. A sample of blood was collected from each rat at 6 and 24 hrs intervals to evaluate hsCRP, TNF-α, IL-6, and IL-10. Rats were sacrificed to get their livers for estimating myeloperoxidase, COX-2, caspase-3, and ALT. Results: A significant difference in the levels of the studied mediators after 6 and 24 hrs was revealed in the test group versus the control group. The correlation analysis showed positive values at both time intervals. ROC analysis demonstrated that the AUC was the highest for TNF-α and hsCRP after 6 and 24 hours, respectively. hsCRP was the only marker that showed an increase in AUC after 24 hrs compared with 6 hours. Conclusion: The levels of the inflammatory biomarkers were increased in LPS-induced acute systemic inflammation up to 24 hrs; also, there was an increase in the sensitivity and specificity for the assessment of acute systemic inflammation.

Keywords: Inflammation, hsCRP, TNF-α, Lipopolysaccharides, COX-2, Caspase-3

Medical Science, 2021, 25(118), 3336-3344

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