Volume 25, Issue 118, December 2021

Organ injury study and the mortality rate assessment in a cecal slurry dose-dependent peritonitis in a rabbit model

Hosien Pormasoumi¹, Daryoush Rostami², Khosro Jamebozorgi¹, Arash Rajabi^3♦

¹Faculty of Medicine, Zabol University of Medical Sciences, Zabol, Iran
²School of Allied Medical Sciences, Zabol University of Medical Sciences, Zabol, Iran
³Trauma Fellowship, Trauma surgery ward, Emdadi Hospital, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran

^♦Corresponding author
Trauma Fellowship, Trauma surgery ward, Emdadi Hospital, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran

ABSTRACT

Introduction: Sepsis is a life-threatening medical crisis in which the body responds to infections extremely. The present study is aimed to assess the extent of mortality in cycle slurry, dose-dependent peritonitis in the rabbit model. Method and Materials: The present study was performed on male polish rabbits. At least 10 days before the experiment, the rabbits were placed in a controlled environment and received standard amounts of water, food, and libitum. Anesthesia of 6 donor rabbits was performed by intramuscular injection of 5 mg/kg Xylasein and 35 mg/kg ketamine. Result: The total number of lymphocytes, neutrophils, and white blood cells (WBC) in the groups of 15 and 20 ml/kg was less than the groups of 5 and 10 ml/kg, no significant differences were observed between the groups (P> 0.05). The number of platelets was also higher in the 20 mL/kg group in regard to the sham group. All of the sepsis groups had lower concentrations of plasma albumin than the sham group. Increased doses of cecal slurry were associated with an increase in the ALT and BUN concentrations. Higher concentrations of ALT and BUN were observed in the sepsis groups than in the sham group. Although the samples in the 15 and 20.0 mL/kg groups showed higher creatinine concentrations than those in the sham group, the difference was insignificant. Conclusion: Based on the DCFH-DA concentrations and blood chemistry analysis, the cecal slurry model used in this study produced dose-dependent, multiple organ injuries and was associated with ROSs generation.

Keywords: Rabbit, Cecal Slurry, Peritonitis, Organ Injury

Medical Science, 2021, 25(118), 3183-3201

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