Volume 25, Issue 115, September 2021

Comparative evaluation of the levels of fibroblast growth factor-2 prepared from Advanced Platelet Rich Fibrin (A-PRF) in diabetic patients and healthy subjects in Saudi Arabia

Hanan Abdullah Mohammed Al Mozher¹, Hesham Abdulatif Al-Mashat², Osamah Mohammed Al Mugeiren^1♦

¹Preventive Dentistry Department, College of Dentistry, Riyadh Elm University, Riyadh, Kingdom of Saudi Arabia
²Department of Periodontology and Implantology, King Saud Medical City, Riyadh, Kingdom of Saudi Arabia

^♦Corresponding author
Preventive Dentistry Department, College of Dentistry, Riyadh Elm University, Riyadh, Kingdom of Saudi Arabia

ABSTRACT

Background: Recently, attempts have been made to alter the centrifugation time in platelet-rich fibrin (PRF) preparation protocols; it was believed that low-speed centrifugation (LSCC) improves growth factor release via PRF scaffolds. Diabetes mellitus is a serious, chronic human metabolic disease that has a tremendous impact on patients, families, and society. The objective of this study was to compare the levels of fibroblast growth factor-2 in advanced platelet-rich fibrin (A-PRF) in diabetic patients and those of healthy subjects from Saudi Arabia. Methods: Blood samples were obtained from diabetic patients at Riyadh Elm University, Saudi Arabia. Eligibility for the participants to be involved in this study was assessed. Samples were obtained from 38 volunteers, 30 patients with type1 and type 2 diabetes mellitus (test group), and eight non-diabetic volunteers (control group). Blood was extracted using 10 mL syringes and then processed using advanced platelet-rich fibrin (A-PRF) centrifugation. A total of 10 milliliters of complete blood without anticoagulant was centrifuged at 1300 rpm (~200 × g) for 14 min at 21–30 °C. Results: Measurements done at four different times (Days 1, 7, 14, and Day 28) revealed that the amount of FGF-2 released from A-PRF was significantly lesser among diabetes mellitus patients than in healthy volunteers. Conclusions: Compared to the non-diabetic individuals, a decrease in the FGF-2 level released from APRF in diabetic patients was observed.

Keywords: diabetes mellitus, fibroblast growth factor, periodontitis, plateletrich fibrin

Medical Science, 2021, 25(115), 2259-2267

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