Volume 25, Issue 109, March, 2021

Antifungal efficacy of clove flower buds (Syzygium aromaticum) oil and aqueous extract against dermatophytes clinical isolates

Mohammed H F Shalayel^1,2♦, Saada Nour³, Ghassab M Al-Mazaideh⁴, Sabry Younis Mahmoud^5,6, Eman Saleh Farrag^7,8

¹Department of Pharmacy Practice, College of Pharmacy, University of Hafr Al-Batin, Hafr Al-Batin, Saudi Arabia
²College of Medicine, Nile University, Sudan
³University of Bahri, College of Medicine, Sudan
⁴Department of Pharmaceutical Chemistry, College of Pharmacy, University of Hafr Al-Batin, Hafr Al-Batin, Saudi Arabia
⁵Biology Department, College of Science, University of Hafr Al-Batin, Hafr Al-Batin, Saudi Arabia
⁶Microbiology Department, Sohag University, Sohag, Egypt
⁷Clinical Laboratory Science Department, College of Applied Medical Science, University of Hafr Al-Batin, Hafr Al-Batin, Saudi Arabia
⁸Microbiology Department, South Valley University, Qena, Egypt

^♦Corresponding author
Department of Pharmacy Practice, College of Pharmacy, University of Hafr Al-Batin, Hafr Al-Batin, Saudi Arabia / College of Medicine, Nile University, Sudan; Email: mhfshalayal@uhb.edu.sa; drmhfs@hotmail.com

ABSTRACT

This study aimed to define the efficacy of clove’s oil and its aqueous extract against dermatophytes clinical isolates in comparison to some antifungal drugs namely, fluconazole, ketoconazole, itraconazole, terbinafine and griseofulvin, by using the in vitro susceptibility test. Terbinafine was found to have the highest potency against all isolates, with an MIC median range of 0.002-0.5 μg/mL. Itraconazole, ketoconazole and griseofulvin revealed comparable good antifungal potency in terms of MIC, 0.05 - 0.5 μg/mL, 0.25 - 0.5 μg/mL, and 0.5 – 1 μg/mL respectively. Meanwhile, fluconazole displayed the least antifungal efficacy (2 - 8 μg/mL). MIC results for Syzygium aromaticum oil and clove aqueous extract were 1.25-2 μg/mL and 125 μg/mL respectively. It was concluded that Syzygium aromaticum oil exhibited a significant antifungal efficacy while, the aqueous extract did not reveal such antifungal potential. This may be due to the hydrophobicity of most of the active ingredients.

Keywords: Clove; Syzygium aromaticum; Dermatophytes; Susceptibility testing; Terbinafine

Medical Science, 2021, 25(109), 744-750

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