Volume 24, Issue 106, November - December, 2020

Exportin-5 gene polymorphism and risk of HCC development in Hepatitis C Egyptian patients

Rabab Fouad^♦, Marwa Wahdan

Department of Hematology, Theodor Bilharz Research Institute, Cairo-Egypt

^♦Corresponding author
Department of Hematology, Theodor Bilharz Research Institute, Cairo, Egypt; Email: dr_rabab_fouad@yahoo.com

ABSTRACT

Background: MicroRNAs (miRNAs) are small non-coding RNAs that play achief role in control of gene expression and contribute in a variety of biological functions. Because of the important role of nuclear export of pre-miRNAs in miRNA biogenesis by exportin-5 (XPO5), any alterations of XPO5 could affect miRNA expression and thus have profound effects on tumorigenesis. Objective: The aim of our work was to examine XPO5 gene polymorphism in HCV- HCC patients compared with HCV positive patients and healthy controls in Egyptian population. Material and methods: Forty nine HCV infected patients, Forty nine patients diagnosed with HCC on top of HCV and 47 healthy volunteers were included in the study. XPO5 gene polymorphism was detected by using RT-PCR (Real Time polymerase chain reaction) technique. Results: A/A, A/C and C/C frequencies in HCV patients were 5%, 22%, 22% respectively, furthermore the frequencies were 3%, 23%, 23% in HCC patients and 6%, 17%, 24% in the control group respectively. The frequencies of A and C alleles in HCV patients were 32 %, 66 % and in HCC patients were 29%, 69% while in the control group the frequencies were 29 %, 65 % respectively. There was no significant difference between the studied groups regarding A/A, A/C, C/C frequencies or A and C allele’s frequencies. Conclusion: XPO5 gene polymorphism is neither associated neither with HCV infection nor with development of Hepatocellular carcinoma (HCC) and its progression.

Keywords: miRNAs, exportin-5 gene polymorphism, HCV and HCC

Medical Science, 2020, 24(106), 4586-4591

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