Objective: the isolation and identification of bacteria in women with clinical vaginosis. Methods: A cross sectional which included 176
women diagnosed with BV according to Amsel criteria. The study carried out in National Cancer Research Center – University of
Baghdad, the participants were collected during the period from February 2019 to December 2019. The participants undergo vaginal
swab and then it was subjected gram stain for bacterial identification. The swab was performed by trained clinicians, in which a
sterile unlubricated speculum was inserted into the vagina then the specimens were collected from the lateral vaginal wall and
posterior fornix using two sterile cotton tipped swabs, the swabs removed carefully to avoid contamination. Results: The study
included 176 women diagnosed with BV, mean age was 31.4 ± 8.9 years, the most common type Staphylococcus aureus with 19%
prevalence, followed by Escherichia coli (16%), 12% Enterobacter spp, 11% Klebsiella pneumonia, 9% and lastly 1% Neisseria spp.
Conclusions: Gram negative bacteria showed the highest rate for causing bacterial vaginosis, only one type of gram-positive bacteria
was present (Staphylococcus) which showed the highest prevalence of all the bacteria.
Keywords: Bacterial vaginosis, microbiota, Gram negative bacteria, contamination