Medical Science
Volume 24, Issue 105, September - October, 2020
Detection of Mycobacterium tuberculosis and drug resistance by GeneXpert® MTB/RIF and Anyplex™ II MTB/MDR in a tertiary care hospital
Reham Hamed Abdel Aziz Yousef1, Nevine Gamal El-din El-Rashidi1, Sahar Mohammed Khairat1, Maha Mohammed Gaafar1, Mohamed Abdel Hakim Elnady2, Mariam Mahmoud Balah1♦, Noha Salah Soliman1
1Clinical and Chemical Pathology Department, Faculty of Medicine, Cairo University, Cairo, Egypt
2Chest diseases Department, Faculty of Medicine, Cairo University, Cairo, Egypt
♦Corresponding author
Mariam Mahmoud Balah,
Department of Clinical and Chemical Pathology,
Faculty of Medicine, Cairo University,
Cairo, Egypt;
E-mail address: dr.mariam_balah@cu.edu.eg
ABSTRACT
2Chest diseases Department, Faculty of Medicine, Cairo University, Cairo, Egypt
Mariam Mahmoud Balah, Department of Clinical and Chemical Pathology, Faculty of Medicine, Cairo University, Cairo, Egypt; E-mail address: dr.mariam_balah@cu.edu.eg
Tuberculosis (TB) is a major global health threat, aggravated by emergence of drug resistance. Early diagnosis is critical and has
been offered lately by molecular assays. This study aimed to evaluate the performance of GeneXpert® MTB/RIF and Anyplex™ II
MTB/MDR assays in simultaneous direct detection of Mycobacterium tuberculosis (MTB) and genetic determination of Rifampicin
(RIF) resistance. This study was conducted with 100 specimens collected from TB suspects. ZN (Zeil-Neelsen) smears were prepared
and L-J (Lowenstein-Jensen) culture was used for isolation of MTB. GeneXpert and Anyplex assays were performed directly on
specimens to detect Mycobacterium tuberculosis (MTB) and RIF resistance. MTB was isolated on L-J culture from 40% of specimens.
For MTB detection, Xpert and Anyplex assays showed same total sensitivity of 95%, and specificities of 73.3% and 81.7%,
respectively. Rifampicin resistance was detected by Xpert and Anyplex assays with rates of 5.5% and 6.12%, respectively showing
significant agreement with a P-value < 0.001. Time of assay was 2 hours for Xpert and 3 hours for Anyplex. In conclusion, both
molecular assays enable rapid concomitant detection of MTB and drug resista
Keywords: MTB; Molecular; Resistance; PCR; Rifampicin; Xpert
Medical Science, 2020, 24(105), 3003-3011
