Volume 24, Issue 105, September - October, 2020

Evaluation of immune factors of blood serum after pulpectomy of dog’s teeth using two root filling material

Leila Basir¹, Zahra Basir², Hadi Naddaf³, Maryam Kouchak⁴, Shabnam Rasti^5♦

¹Associate Professor, pediatric department, school of dentistry, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
²Assistant Professor, Department of Basic Science, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran.
³Professor, Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran.
⁴Professor, Nanotechnology Research Center and Department of Pharmaceutics, School of Pharmacy, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
⁵Pediatric dentistry Resident, Department of Pediatric Dentistry, School of dentistry, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran

^♦Corresponding author
Pediatric dentistry Resident, Department of Pediatric Dentistry, School of dentistry, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran

ABSTRACT

Background and Purpose: This study aimed to evaluate the immune factors of blood serum (IgG, IgM, and IgE serum levels and complement components C3 and C4 and blood cells after pulpectomy of dog's teeth using two root fillers (ZOE and Chitosan). Method: The present study is a semi-experimental study conducted on 40 root canals in two groups (20 channels in 2 dogs for Chitosan and 20 channels equal to 2 dogs for ZOE). The healthy teeth of the first, second and third premolars of the lower and upper jaw (40 canals) in 4 dogs aged 12-18 months were selected for the treatment of pulpectomy. Before treatment and after 28 days, 5 cc of blood was taken from the dogs and the serum immune factors were studied. The mean values obtained from the two groups were compared using T-test. Data analysis was performed using SPSS software version 22. Findings: The rate of WBC changes between the two treatment groups with ZOE has increased compared to Chitosan (P = 0.03). The levels of C3 and C4 decreased more due to the use of ZOE than chitosan. The rate of IgG changes with ZOE increased compared to chitosan. The rate of change in IgM and IgE between the two treatments groups with ZOE was not significant compared to Chitosan. IgE levels in the chitosantreated group did not change significantly (P> 0.9). The rate of IgE changes between the two treatment groups with ZOE and chitosan was not significant (P = 0.65). There was no change in the serum level of factors associated with red blood cells, including, number of red blood cells, HCT, MCV, MCH, and MCHC. The rate of albumin and total protein changes between the two treatment groups with ZOE was not significant compared to chitosan. Conclusion: In comparison between two root canal material, ZOE increased white blood cells and immunoglobulins. There was no difference in blood factors and protein levels between the two groups.

Keywords: Immunoglobulins, Complement, Dental pulpectomy, ZOE, Chitosan

Medical Science, 2020, 24(105), 3602-3613

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