Introduction: Entamoeba histolytica is belonging to the family of Entamoebidae and phylum Amoeboza. There are two forms of E.
histolytica – trophozite and cyst. It is 10 to 50μmin diameter. Trophozoites ingest erythrocytes and destruct the tissue; cyst forms
ulcers in the colon. E. histolytica causes amoebiasis commonly in travellers, recent immigrants, homosexual men, and pregnant
women. It is found in the tropical and subtropical area and around 1 lakh people die due to amoebiasis all over the world. In India,
the infection is present in most of the rural places, where the poor sanitation presents. Case presentation: A case of amoebiasis is
found in pregnant women residing in villages of district nearby Wardha, in Maharashtra. Enrolled pregnant women complained
about bloody diarrhea, vomiting, fever, etc. The purpose of this study is to be aware of amoebiasis infection in pregnancy due to the
consumption of unhealthy water and food. In the primary level, microscopy of a patient stool sample is very useful for the diagnosis
of disease caused by E. histolytica parasite. Conclusion: In this study, we mainly focus on molecular diagnostic method i.e. Real-time
PCR. It affects people other than pregnant women, but it is severe in the pregnant woman because preterm delivery occurs due to
amoebiasis. Due to immunodeficiency in pregnancy time, amoebiasis becomes severe in pregnant women.
Keywords: Amoebiasis, RT-PCR, Pregnant woman, Sanitation, Fecal.