Medical Science
Volume 24, Issue 103, May - June, 2020
Protective effect of royal jelly on Busulfan-induced liver injury in male Wistar rats
Tayebeh Sadeghi1, Seyed Ebrahim Hosseini1♦, Mohammad Amin Edalat Manesh1, Mohammad Khaksari2
1Department of Biology, Shiraz branch, Islamic Azad University, Shiraz, Iran
2Department of Physiology and Pharmacology, Kerman University of Medical Sciences, Kerman, Iran
♦Corresponding author
Department of Biology, Shiraz branch, Islamic Azad University, Shiraz,
Iran;
Email: ebrahim.hossini@yahoo.com
ABSTRACT
Busulfan is a cancer drug in chemotherapy that may inflict damages to various tissues, especially the liver as the major site of drug
filtration and monitoring due to its alkylating and DNA adhesion properties and disrupting its operation causing Hepatic enzymes
increase as one of the key indicators of liver tissue damage recognition. Given the importance and efficacy of royal jelly in reducing
tissue stress and healing induced by Busulfan, the current paper aimed at evaluating the effect of royal jelly on liver enzymes of Male
Wistar rats received Busulfan. The current paper examined 60Male Wistar rats aged 2.5 to 3 months with an approximate weight of
200-220 g in 4 groups of 15 each. The grouping was as follows: the control group received subcutaneous injection of distilled water
and the two control groups received a 10 mg/kg Busulfan in the first and the 21 days of the experiment and 35 days after the first
injection they were given 100 mg/ kg royal jelly for 56 days by gavage. In the other group, rats received only royal jelly at a dose of
100 mg / kg for 56 days by gavage. Five rats in each group were anesthetized on days 14, 28, 56 after injection, and serum levels of
liver enzymes were measured after cardiac bleeding. One-way analysis of variance was used for data analysis and p <0.05 was
considered as the level of significance. The results of this study showed that treatment with royal jelly had the most significant
decrease in serum levels of AST and ALP liver transaminases. In contrast, Busulfan treatment group showed the highest degradation
and increased serum levels of AST and ALP liver transaminases (P≤0.05). Also, royal jelly reduced the activity of liver AST and ALP
transaminases in adult male rats treated with Busulfan which significantly decreased with the continuation of the treatment period
and at the 56-day measurement phase. Overall (p <0.05), the final result showed that royal jelly can be used to reduce the damaging
effects of Busulfan on liver tissue.
Keywords: Busulfan, Royal jelly, Liver enzymes, Wistar rats
Medical Science, 2020, 24(103), 1427-1432
