Statement of the Problem: Several improvements in dental adhesive technology and advances in bonding knowledge have been
achieved, but the issue of bond durability between resin composite and dentine is still a major challenge in adhesive dentistry.
Purpose: The aim of the present study was to evaluate the effect of chlorhexidine (2% inhibitor matrix metallo proteinase) and
collagen cross-linking agent (Quercus extract for stabilizer of collagen fibers) on tensile bond strength of composite to dentin.
Materials and Method: A total of 75 extracted human upper premolar teeth were selected for the study. The samples were stored in
0.5% thymol solution. Then, the occlusal enamel was removed and the surface of dentine was etched by phosphoric acid and rinsed
with distilled water for 30 s and air dried. The specimens were divided into five groups as follow: chlorhexidine, Quercus extract,
chlorhexidine+ Quercus extract, Methanol (solvent of Quercus extract), and control group (without applying specific solution).
Following the etch-and-rinse adhesive, the resin composite was placed on the dentin surface. The micro-tensile bond strength
(μTBS) of the specimens after three months water storage and 1500-cycle thermocycling was examined. Data were analysed using
Multiple T-tests, ANOVA, and SPSS ver.20. Results: Variance analysis and t test showed that the Quercus extract group had the
highest bond strength compared to control group, but the difference was not significant. Chlorhexidine showed negative effects on
the composite-dentin bond strength. Conclusion: The clinical application of 2% chlorhexidine and Quercus extract could not increase
the tensile bond strength of composite resin to dentine.
Keywords: Chlorhexidine, Collagen cross-linking, Microtensile bond strength, Quercus extract