Volume 24, Issue 102, March - April, 2020

Comparison of two microscopic methods for the detection of parasitic infection in antenatal women in selected areas

Anil Tambekar¹, Nishant Burnase², Abhay Gaidhane³, Punit Fulzele⁴, Zahiruddin Quazi Syed ^5♦

¹M.Sc. Microbiology, Junior Research fellow, Research & Development, Datta Meghe Institute of Medical Sciences, Sawangi (M), Wardha; Email: anil.m.tambekar11@gmail.com
²M.Sc. Biotechnology, Junior Research Fellow, Research & Development, Datta Meghe Institute of Medical Sciences, Sawangi (M), Wardha; Email: nishantburnase@gmail.com
³M.B.B.S. MD (Community Medicine), School of Epidemiology and Public Health, Datta Meghe Institute of Medical Sciences, Sawangi (M), Wardha.; Email: abhaygaidhane@gmail.com
⁴Master of Dental Surgery, Associate Professor, Department of Pedodontics, Sharad Pawar Dental College & Hospital, Sawangi (M), Wardha; Email: punitr007@gmail.com
⁵M.B.B.S. MD (Community Medicine), Ph.D. (Community Medicine). School of Epidemiology and Public Health, Datta Meghe Institute of Medical Sciences, Sawangi (M), Wardha.; Email: zahirquazi@gmail.com

^♦Corresponding author
M.B.B.S. MD (Community Medicine), Ph.D. (Community Medicine). School of Epidemiology and Public Health, Datta Meghe Institute of Medical Sciences, Sawangi (M), Wardha Email: zahirquazi@gmail.com

ABSTRACT

Background: Soil-transmitted helminths infections are a major health problem all around the globe. According to WHO 3.5 billion people, affect and 2 million people caused morbidity (Lohe et al., 2010). In Maharashtra state, a low level of hygienic practices, bad quality of water supplies is responsible for parasitic infection. The microscopic examination of stool specimens was essential for the diagnosis of intestinal parasites (Basak, Singh, and Rajurkar, 2016). Light microscopy is typically performed in the laboratory settings far from where most STH- infected person resides, but the new diagnostic tool is invented known as foldscope(Khanam et al. 2019) and (M. Khatib et al., 2018). Foldscope is portable and easy to use in the field. Methodology: The study was carried out in Maharashtra, India. Total 170 antenatal women were enrolled and out of which 166 stool sample were collected. 166 stool samples were examined by iodine and saline mount under Foldscope and conventional Microscope. Result: Of the 166 sample, 15 sample positive for soil-transmitted helminth in conventional microscopy and 6 sample positive for foldscope microscopy. Conclusion: Conventional microscopy was found to be the most sensitive method for the diagnosis of soil-transmitted helminth parasites. Foldscope require improvement in magnification.

Keywords: Antenatal women, parasites, foldscope, microscopy.

Medical Science, 2020, 24(102), 557-564

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