Volume 24, Issue 101, January - February, 2020

Investigation of the effect of L-Carnitine on Mouse Ovarian Transplantation

Fatemeh Shahi Sadrabadi¹, Hussein Eimani², Kazem Parivar^1♦, Abdolhussein Shahverdi²

¹Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran
²Department of Embryology, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran

^♦Corresponding author
Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran Email: Kazem_parivar@yahoo.com

ABSTRACT

Introduction: L-carnitine (LC) has yielded relevant results on ischaemic/ reperfusion (I/R) injuries in organs such as the intestine, liver, kidney, brain and heart, because of its antioxidant activity. We aimed to evaluate the potential of using L-carnitine to improve the quality of grafted ovarian tissues. Materials and methods: The ovaries from 4-6-week old mice were grafted into back muscle site. Transplanted ovaries were divided into four groups. 200 mg/kg LC was injected intraperitoneally one day before surgical operation and repeated until one week after grafting. Follicular morphology and apoptosis were examined in all groups and compared to agematched non grafted ovaries. Anti- active caspase-3 staining was performed to evaluate apoptosis in all groups. Results: group Morphological analysis revealed that the number of follicles decreased in all transplanted groups compared with non-grafted group. The rate of apoptosis in LC- treated group showed no significant differences compared with control. Conclusion: LC supplementation may be a suitable approach for the ovarian graft viability. Further experimental studies are required to clarify the mechanisms of action of L-carnitine, and clinical studies are required to determine optimum dose and treatment duration for decreasing I/ R injuries.

Keywords: Apoptosis, Follicle, L-carnitine, ovarian transplantation

Medical Science, 2020, 24(101), 343-350

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