Volume 24, Issue 101, January - February, 2020

Renoprotective influence of Teucrium polium leaf extract on Rats Intoxicated by Cyclophosphamide

Manal MS Mansoury

Food and Nutrition Department, Faculty of Home Economics, King Abdulaziz University, Jeddah, Saudi Arabia

ABSTRACT

Teucrium polium leaves extract (TPLE) used as traditional herbal therapy for various diseases. Long term treated with Cyclophosphamide (CD) concomitant with development of renaltoxicity. The present study aims to assess the mechaism of renal protective effect of TPLE on rats intoxicated with CD. Forty male rats were separated into 4 groups; control (Cont), CD; rats intraperitoneal (i.p) injected with CD (fifty mg/kg b.wt) for twenty one days, TPLE two hundred mg/kg b.wt +CD and TPLE four hundred mg/kg b.wt +CD; rats received TPLE orally for 21 days, followed by i.p. injected with CD. Following 24 hours of the last dose of CD blood and renal samples were collected for biochemical and histopathogical investigations. Renal oxidative stress biomarkers; lipid peroxides (MDA) and superoxide dismutase (SOD), serum anti-inflammatory biomarkers, tumor necrosis factor-alpha (TNF-α) and interleukin-1beta (IL-1β), kideny function; serum levels of creatinine(Cr), urea (BUN) and uric acid (UA), as well as serum ionic levels of sodium (Na+) and potassim (K+) were derermined. The current study demonestrated that injection of CD caused significant increases in renal MDA, serum anti-inflammatory cytokines (TNF-α and IL- 1β), serum kidney function paramters and the serum ionic K+ levels, with significant decreases in the renal SOD and serumionic Na+ against control group. Renal tisuues showing congestion and coagulating necrosis of the renal tubules in the CD group. Oral adminstration of TPLE significantly ameliorated CD-induced renal oxidative stress. It reduced CD-induced elevation in serum antiinflammatory cytokines, kidney function parameters and the changes in ionic Na+ and K+ levelsas well as it ameliorate the changes in kidney tissues compared with CD group. Therefore, TPLE ameliorates the effect of CD-induced nephrotoxicity.

Keywords: Teucrium polium leaf; cyclophosphamide; renaltoxicity; antioxidant; anti-inflammatory.

Medical Science, 2020, 24(101), 249-258

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