Volume 24, Issue 101, January - February, 2020

Growth kinetic comparison of Human Mesenchymal Stem Cells from Bone Marrow, Adipose Tissue and Decidua

Sepide Kazemi¹, Kazem Parivar^1♦, Nasim Hayati Roudbari¹, Parichehr Yaghmaei¹, Behnam Sadeghi²

¹Department of Biology, Faculty of Basic Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran
²Immunotherapy and Regenerative Medicine Department, Breast Cancer Research Center, Motamed Cancer Institute, ACECR, Tehran, Iran

^♦Corresponding author
Professor, Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran

ABSTRACT

Introduction: Mesenchymal stromal cells (MSCs) are undifferentiated cells with high proliferation potency. These cells can be characterized by their ability to self-renew and differentiate into specialized cell lineages. Therefore, these cells are considered as an attractive source of stem cells for cell therapy. However, MSCs are slightly different from different sources. The most used stem cell types, in both human and veterinary fields, are MSC derived from bone marrow and adipose tissue. Nowadays, there is a great interest in using stem cells derived from fetal tissues, such as decidua, which can be obtained non-invasively at delivery time and are an unlimited source of stromal cells. Materials and methods: In this study, biological characteristics of stromal cells from adipose tissue (AT-MSC), bone marrow (BM_MSCs), and decidua (DSC), such as proliferation capacity, passage capacity, colony formation and cell viability after cryopreservation were compared. Results: Based on our results, population doubling time of DSC in comparison with BM_MSC and AT-MSC is shorter (under 20 hours to passage 10), and these cells possess the highest potential in colony formation and ability of self-renewal. Also, these cells were well-maintained their viability after Cryopreservation up to 80%. Conclusion: DSCs have higher colony-forming ability and long-time passage capacity. Due to the significant proliferation capacity and great viability after cryopreservation, DSCs may be considered as a new source of stromal cells for application in cell-based therapies.

Keywords: Mesenchymal stem cell, Decidua, Cryopreservation, Proliferation capacity, Colony formation.

Medical Science, 2020, 24(101), 223-234

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