Volume 24, Issue 101, January - February, 2020

A comparison of the cytotoxic activity of cisplatin versus L-amino acid oxidase on squamous cell carcinoma cell line

Wael Y. Elias

Associate Professor and Chairman of Oral Diagnostic Science Department, Faculty of Dentistry, King Abdulaziz University, Jeddah, Saudi Arabia, Email: dreliasw@gmail.com

ABSTRACT

Objectives: This study used flowcytometry to compare the cytotoxic effect of cisplatin and L-amino acid oxidases (LAAOs) on the HEP-2 cell line 24- and 48-hours following treatment. Methods: This prospective laboratory study was conducted at the Applied Research Unit, Vaccine and Serum Research Institute, Cairo, Egypt and King Abdulaziz University- faculty of dentistry, Jeddah, Saudi Arabia in July 2017.The HEP-2 cell line was cultivated and cisplatin and LAAO were added to the culture medium. Flowcytometry was used to assess the cells 24 and 48 hours following treatment. Results: We found that LAAOs had a greater cytotoxic effect than cisplatin against the HEP-2 cell line, compared to cisplatin. The apoptosis rate was directly proportional to the duration of exposure. Conclusions: Our analyses show that LAAO has a stronger cytotoxic effect on HEP-2 cells than cisplatin, highlighting the potential of LAAO in the treatment of squamous cell carcinoma. Although our findings still need to be validated in other models, our findings strongly suggest that LAAO may cause substantial tissue damage.

Keywords: Squamous cell carcinoma cell line, HEP-2, Cisplatin, L-aminoacid oxidase LAAO, Flowcytometry

Medical Science, 2020, 24(101), 183-189

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