Volume 18, Issue 41, January - June, 2024

Titrimetric and thin layer chromatographic fingerprint analysis of captopril solid dosage form – an Angiotensin- Converting Enzyme Inhibitor

Samuel J Bunu¹, Somtochukwu Kela−Eke², Benjamin U Ebeshi²

¹Department of Pharmaceutical and Medicinal Chemistry, Faculty of Pharmacy, Niger Delta University, Wilberforce Island, Bayelsa State, Nigeria
²Department Of Pharmaceutical Chemistry, Faculty of Pharmacy, Madonna University, Elele Campus, Elele, Nigeria

ABSTRACT

Captopril is an angiotensin-converting enzyme inhibitor (ACEI), that prevents the conversion of Angiotensin I to Angiotensin II, thereby decreasing blood pressure by lowering peripheral vascular pressure without increasing cardiac output or contractibility. 25 mg of 20 Captopril tablets from five brands (ACEI1-ACEI5) were weighed and pulverized into a fine powder. 100 mg of the powdered tablet active component was combined with 60 mL of water in a 100 mL measuring cylinder and thoroughly shaken for 20 minutes to extract the medicament. The solution will then be filtered. Titration was conducted by adding 2 mL of 5M HCl to 10 mL aliquots of captopril, 0.02 M Hexacyanoferrate (III), and 30% ZnSO4. For the titrimetric study, the excess oxidant was iodometrically measured in the presence of ZnSO4. Thin Layer Chromatographic Fingerprinting was done following pharmacopeia standards, compared with 5.0 mL of methanol and 10 mg captopril tablet powder. The blank titrimetric analysis of thiosulphate without the captopril samples gave 48.3 ml, and test samples ranging from 48.4 ml (ACEI2 and ACEI4), 48.5 ml (ACEI1) and 48.6 ml (ACEI3 and ACEI5), respectively. The TLC, for ACEI1, ACEI3, and ACEI6, under 254 nm, showed a violet color, and blue at 365nm, indicating the presence of captopril, with mean Rf values of 0.70, 0.71 and 0.70 cm respectively. Both methods showed the presence of captopril and an adequate amount of captopril in the tablet used for the analysis. Hence, these methods may be useful in routine captopril analysis.

Keywords: Captopril, TLC, Titrimetric, ACE Inhibitor, Chromatographic Fingerprint

Drug Discovery, 2024, 18(41), e5dd1964

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DOI: https://doi.org/10.54905/disssi.v18i41.e5dd1964

Published: 28 January 2024