Volume 17, Issue 40, July - December, 2023

Effect of receptor and donor phase composition on permeation of pharmaceutical compound through model membrane

Muhmmad Abid Mustafa^1♦, Mahnoor Rashid¹, Shakeel Ahmad¹, Hamza Farooq², Asad Majeed Khan¹, Saba Naeem¹, Barira Amin¹, Fahad Ahmed³, Qandeel Rafi¹, Nurhan Tariq¹

¹Department of Pharmaceutics, Lahore Pharmacy College, Lahore Medical & Dental College, University of Health Sciences, Lahore, Pakistan
²Research Student, Lahore Pharmacy College, Lahore Medical & Dental College, University of Health Sciences, Lahore, Pakistan
³Faculty of Pharmacy, University of Sargodha, Sargodha, Pakistan

^♦Corresponding author
Department of Pharmaceutics, Lahore Pharmacy College, Lahore Medical & Dental College, University of Health Sciences, Lahore, Pakistan

ABSTRACT

Background: Emerging interest in the development of nanotechnology liposomes to encapsulate, protect and deliver lipophilic components i.e., nutraceuticals, drugs, flavors, antioxidants and antimicrobial agents has revolutionized food, pharmaceuticals, agrochemicals and other industries. Method: Permeation of salicylic acid through a silicon membrane (the model membrane) was examined by using different compositions of buffer and propylene glycol. Franz cells were used to study the penetration of salicylic acid through a silicon membrane. The two buffer solutions of 7.5 and 6.8 pH were prepared. Afterwards, utilizing pH 7.5 buffers, four combinations of concentrations were prepared: 100% buffer, 75% buffer with 25% propylene glycol (3:1), 25% buffer with 75% PG (1:3) and 50% buffer with 50% PG (1:1). Similarly, four different concentrations were prepared with a buffer of 6.8 pH (same as above). Absorption of drugs in the receptor cell medium was investigated by measuring absorbances using a UV/vis spectrophotometer. Result: A total of eight results were obtained and compared to investigate the concentration at which salicylic acid absorbs most effectively. At pH 6.8, the absorbance was 31.1%; at (3:1) buffer: PG, 69.9%; at (1:1), 77.2%; and at (1:3), 58.8%. At pH 7.5, the absorbance was 24.5%; at (3:1) buffer: PG, 46.5%; at (1:1), 53.4%; and at (1:3), 22.2%, measured at a final test time of 75 minutes. Out of these four concentrations, salicylic acid showed maximum absorption at (1:1) buffer: PG combination. In pH 6.8 buffer, the salicylic acid showed more absorption and release through the membrane as compared to pH 7.5 buffer. The results were compared and discussed accordingly. Conclusion: A permeation study of salicylic acid through a silicon membrane indicates that both buffer and propylene glycol play a crucial role in absorption. The ratio of combination and pH has an essential influence upon absorption. From the results, it was inferred that salicylic acid showed maximum absorption, permeation and release through the membrane at an acidic pH of 6.8. It means that if in transdermal drug delivery the pH of the skin is lower, salicylic acid will present enhanced permeation.

Keywords: Salicylic acid, Propylene glycol, Phosphate Buffer, Silicon membrane, Franz cell, FTIR, diffusion, absorbance, in-vivo, ex-vivo, permeation

Drug Discovery, 2023, 17(40), e27dd1943

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DOI: https://doi.org/10.54905/disssi.v17i40.e27dd1943

Published: 07 July 2023