Volume 17, Issue 39, January - June, 2023

Population genetics and diversity of human adenovirus in two tertiary hospitals in Southern Nigeria

Dirisu John^1♦, Agbakoba Nneka², Eki-Udoko Fidelis³, Elo- Ilo Jacinta C⁴, Oladipo Olukayode⁵, Okwelogu Somadina⁶

¹Department of Medical Microbiology, Faculty of Medical Laboratory Science, Ambrose Alli University, Ekpoma, Nigeria
²Department of Medical Laboratory Science, Faculty of Health Sciences, Nnamdi Azikiwe University, Nigeria
³Department of Paediatrics, University of Benin Teaching Hospital, Benin City, Nigeria
⁴Department of Paediatrics, Nnamdi Azikiwe University Teaching Hospital, Nnewi, Nigeria
⁵Institute of Child Health, University of Benin, Benin City, Nigeria
⁶Department of Parasitology and Entomology, Nnamdi Azikiwe University, Awka, Nigeria

^♦Corresponding author
Department of Medical Microbiology, Faculty of Medical Laboratory Science, Ambrose Alli University, Ekpoma Nigeria

ABSTRACT

The study of dynamic changes in genetic heterogeneity can shed light on the evolution of viruses because it is known that the genetic variety of viral populations within their hosts affects disease progression, treatment outcome, medication resistance, cell tropism and transmission risk. Our study's primary goal is to identify trends in the genetic makeup and variety of human adenoviruses using samples taken from children who attend two distinct tertiary hospitals in Southern Nigeria's Benin City and Nnewi. In Benin City and Nnewi, 330 pediatric patients' samples from tertiary hospitals were gathered and after DNA extraction and Sanger sequencing, the samples were examined. The obtained genetic information matrix was used to calculate the Jaccard similarity index, genetic distance and genetic diversity studies. According to the Analysis of Molecular Variance (AMOVA), this variation was more evenly distributed within each site's population (97%) than it was between them (3%). The effective number of alleles Ne, which represents genetic diversity at the population level, was 2.358 for Benin City and 2.255 for Nnewi, respectively. This shows that each of the studied loci had an average of 2.358 and 2.255 alleles per locus in these two populations. As a result, Nnewi has less genetic diversity of human adenovirus than Benin.

Keywords: Genetic diversity, human adenovirus, AMOVA, loci, children, population

Drug Discovery, 2023, 17(39), e6dd1007

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DOI: https://doi.org/10.54905/disssi.v17i39.e6dd1007

Published: 06 February 2023