Volume 17, Issue 39, January - June, 2023

Characterization of bioactive compounds in Luffa aegptiaca leaf ethanolic extracts using gas chromatography and mass spectrometry

Alagbe JO^1♦, Bamigboye Samson², GC Nwosu², DA Agbonika³, Kadiri Mercy Cincinsoko⁴

¹Department of Animal Nutrition and Biochemistry, Sumitra Research Institute, Gujarat, India
²Department of Animal Science, University of Abuja, Nigeria
³Department of Agricultural Economics, University of Abuja, Nigeria
⁴Kazachat College Abuja, Nigeria

^♦Corresponding author
Department of Animal Nutrition and Biochemistry, Sumitra Research Institute, Gujarat India
ORCID ID: 0000-0003-0853-6144

ABSTRACT

Medicinal plants contain several bioactive compounds or phytochemicals that therapeutically exert wide spectrum of biological activities such as; antimicrobial, antifungal, antiviral, anti-inflammatory, hepato-protective, hypolipidemic, analgesics, antioxidant, immune-stimulatory, immune-modulatory and anticancer. These bioactive compounds are effective, non-toxic and environmental friendly. The aim of this experiment was to determine the bioactive compounds in Luffa aegptiaca ethanolic leaf extract using gas chromatography and mass spectrometry (GC-MS). Phytochemical analysis of the sample reveals the presence of alkaloids (1314.9 mg/g), glycosides (11.60 mg/g) while tannins, flavonoids, terpenoids, steroids, saponins and oxalates were present in 807.56 mg/g, 512.9 mg/g, 308.7 mg/g, 192.6 mg/g, 102.1 mg/g, 61.44 mg/g and 20.90 mg/g respectively. GC-MS analysis of Luffa aegptiaca ethanolic leaf extract shows that it contains 35 bioactive compounds with marked pharmaceutical properties. 1,11-Bicyclopropyl, 2-octanoic acid had the highest concentration (19.06 %) while β-methylnaphthalene had the lowest concentration (0.01%). It was concluded that the presence of these phytochemicals will inhibit some pathogenic bacteria’s thus promoting a healthy gut in animals.

Keywords: Luffa aegptiaca extracts, medicinal plants, phytochemicals, gas chromatography, mass spectrometry

Drug Discovery, 2023, 17(39), e10dd1011

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DOI: https://doi.org/10.54905/disssi.v17i39.e10dd1011

Published: 16 February 2023