Volume 15, Issue 36, July - December, 2021

In-Silico Antifungal Drug Discovery for Mucormycosis Targeting β- Glucan Synthase

Shahi A¹, Mishra SK²

¹Post graduate, Department of Biotechnology, Deen Dayal Upadhyaya Gorakhpur University, Gorakhpur, Uttar Pradesh, India
²Department of Biotechnology, Deen Dayal Upadhyaya Gorakhpur University, Gorakhpur, Uttar Pradesh, India

ABSTRACT

Phytochemical constituents of medicinal plants are being used in the management of liver disease and in various pathological states. In this present study, we evaluated the mitigating effect of peels of fruit of C. tangerina against CCl4-induced hepatotoxicity in pre-treated Wistar rats. Rats (180 – 220 g) were randomly placed into five groups of five animals each. Group 1 served as normal control, group 2 received CCl4, group 3 was silymarintreated (standard), while groups 4 and 5 received Citrus tangerina peels extract (CTP) at 200 mg/kg and 400 mg/kg. CTP and silymarin were administered orally for six days while CCl4 was given subcutaneously on the 7th day only. Following euthanasia, blood samples were collected used for the estimation of biochemical parameters namely superoxide dismutase (SOD), catalase (CAT), malondialdehyde (MDA), alkaline phosphatase (ALP), aspartate aminotransefrase (AST), and alanine transaminase (ALT). The liver tissues harvested were subjected to histopathology. Statistical analysis was done and P<0.05 were considered significant. CTP significantly (P<0.05) reduced elevated AST caused by CCl4 intoxication. CCl4 induced decrease in antioxidant enzymes SOD and CAT were elevated by both doses of CTP as much as silymarin. Histopathological observation of the liver tissue supported biochemical findings of this study. Methanol extracts of C. tangerina fruit peels possess potential protective activity on the liver in CCl4-induced hepatotoxicity.

Keywords:Drug discovery, Molecular docking, Mucormycosis, Black fungus, Plants-derived phytochemicals, Glucan synthase

Drug Discovery, 2021, 15(36), 221-230

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