Volume 15, Issue 36, July - December, 2021

Citrus Tangerina pre-treatment mitigates carbon tetrachloride induced hepatotoxicity on Wistar rats

Moke EG^1♦, Eduviere AT¹, Anachuna KK², Nwogueze BC², Erhenhi AH³, Asiwe JN⁴, Omorodion LI¹

¹Department of Pharmacology and Therapeutics, Faculty of Basic Medical Sciences, Delta State University, Abraka, Nigeria
²Department of Physiology, Faculty of Basic Medical Sciences, Delta State University, Abraka, Nigeria
³Department of Botany, Faculty of Sciences, Delta State University, Abraka, Nigeria
⁴Department of Physiology, PAMO University of Medical Sciences, PortHarcourt, Nigeria

^♦Corresponding author
Emuesiri Goodies Moke, Department of Pharmacology and Therapeutics, Faculty of Basic Medical Sciences, Delta State University, Abraka, Nigeria

ABSTRACT

Phytochemical constituents of medicinal plants are being used in the management of liver disease and in various pathological states. In this present study, we evaluated the mitigating effect of peels of fruit of C. tangerina against CCl4-induced hepatotoxicity in pre-treated Wistar rats. Rats (180 – 220 g) were randomly placed into five groups of five animals each. Group 1 served as normal control, group 2 received CCl4, group 3 was silymarintreated (standard), while groups 4 and 5 received Citrus tangerina peels extract (CTP) at 200 mg/kg and 400 mg/kg. CTP and silymarin were administered orally for six days while CCl4 was given subcutaneously on the 7th day only. Following euthanasia, blood samples were collected used for the estimation of biochemical parameters namely superoxide dismutase (SOD), catalase (CAT), malondialdehyde (MDA), alkaline phosphatase (ALP), aspartate aminotransefrase (AST), and alanine transaminase (ALT). The liver tissues harvested were subjected to histopathology. Statistical analysis was done and P<0.05 were considered significant. CTP significantly (P<0.05) reduced elevated AST caused by CCl4 intoxication. CCl4 induced decrease in antioxidant enzymes SOD and CAT were elevated by both doses of CTP as much as silymarin. Histopathological observation of the liver tissue supported biochemical findings of this study. Methanol extracts of C. tangerina fruit peels possess potential protective activity on the liver in CCl4-induced hepatotoxicity.

Keywords: Antioxidant, Citrus tangerina, hepatic cell protection, methanol extract.

Drug Discovery, 2021, 15(36), 215-220

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