Volume 15, Issue 36, July - December, 2021

Synthesis and isolation of ion association complex of Bupivacaine HCl and Its Spectrophotometric Estimation in bulk and pharmaceutical Preparations

Munan A^1,2♦, Waseem AA², Rahman SF², khan AJ^1,3, Khan M^1,4, Abbas G¹, Iqbal A^1,2, khan O^1,5, Khan S¹

¹Ophth Pharma (Pvt) Ltd, Pakistan
²Department of Chemistry University of Karachi, Pakistan
³Sindh Institute of Urology and Transplant, Pakistan
⁴Layton Rahmatullah Benevolent Trust Eye Hospital Korangi Karachi, Pakistan
⁵Department of Pharmacy University of Karachi, Pakistan

^♦Corresponding author
Ophth Pharma (Pvt) Ltd, Pakistan / Department of Chemistry University of Karachi, Pakistan

ABSTRACT

A simple spectrophotometric method was developed by using the picric acid as an ion pair reagent with Bupivacaine HCl (BH) in bulk and injection dosage forms. The ion-Association complex formed between BH and PA was extracted with chloroform and identified its reaction sites by FTIR. The extracted yellow colored solution of complex showed maximum absorbance at 410 nm wave length. The bear and lambert law was obeyed at range of 10-100 ppm. The limit of detection, Limit of Quantification, slop and regression coefficient (r2) was respectively 5μg, 10-20μg, 0.455 and 0.9998. The developed and validated analytical method was successfully applied for the quantitation of pharmaceutical preparations. The recoveries and low standard deviations confirm the suitability of spiked analytical method.

Keywords: Ion-association complex, Bupivacaine HCL (BH), picric acid (PA), Validation, Stability

Drug Discovery, 2021, 15(36), 159-168

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