Volume 15, Issue 35, January - June, 2021

A novel validated method for estimation of Carbachol in ophthalmic preparations by spectrophotometer

Abdul Munan^1,2♦, Akhtar Jamal Khan^1,3, Mahwash Khan^1,4, Abdul Haleem^1,5, Muhammad Ishaq Nazeer¹

¹Ophth Pharma (Pvt) Ltd, Pakistan
²Department of Chemistry, University of Karachi, Pakistan
³Sindh Institute of Urology and Transplant, Pakistan
⁴Layton Rahmatullah Benevolent Trust Eye Hospital, Korangi, Karachi, Pakistan
⁵Department of Pharmacy, Baqai University, Karachi, Pakistan

^♦Corresponding author
Ophth Pharma (Pvt) Ltd, Pakistan; Email: abdulmunan17@gmail.com

ABSTRACT

A simple and selective novel spectrophotometric method has been proposed for determination of Carbachol in pure and pharmaceutical ophthalmic preparations. The method is developed on formation of ion-pair complex between Bromophenol blue (BPB) and Carbachol (Carb), which is blue in color. The variables which effect the complex formation were thoroughly studied and optimized. The Beer’s and Lambert’s plots were obeyed in a concentration range of 10-100 ppm with regression co-efficient 0.9999. The LOD and LOQ are obtained respectively 5ppm and 10ppm. The proposed method was successfully applied to the analysis of the cited drugs in ophthalmic preparations. The Linearity, precision, specificity, robustness and ruggedness of proposed method were evaluated as per ICH and USP guidelines.

Keywords: BPB (Bromophenol blue), Quaternary Ammonium, Ion-Pairing, Method validation

Drug Discovery, 2021, 15(35), 43-51

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