Volume 14, Issue 34, July - December, 2020

Antitumor activity of plant associated endophytic actinomycetes

Kanchanadevi D, Hemashenpagam N

Assistant Professor, PG and Research Department of Microbiology, Hindusthan college of Arts and Science, Coimbatore-28, India

ABSTRACT

The current analysis segregated the endophytic actinomycetes from bio-materials like cabbage (Brassica oleraecea), tomato (Solanum lycopersicum) and maize (Zea mays). The total 40 actinomycetes were isolated using Starch casein agar. The huge anti-bacterial action found in 4 (10%) endophytes among forty endophytic actinomycete samples. They were recovered using agar streak method on Starch casein agar. The production of antimicrobial compound was performed by fermenting ISP 2 broth with 4 isolates for 15 days. After fermentation, extraction of the supernatant was carried out using solvent ethyl acetate. Analysis of 16S rDNA sequencing of those isolates showed that strain 34 belong to member of the genera Streptomyces sampsonii. Column & TLC Chromatography techniques were utilized to recover the anti-bacterial analogs. The HPLC-UV chromatogram of the extracts exhibited most exciting diversity of the chemical constituents. Crude extract of S34 exhibited three different peaks at retention time (RT) 1.905, 2.608 and 3.027. Investigation of tumor selectivity of the 4 endophytic actinomycete metabolites against selected Breast cancer cell lines indicated effective and promising differential cytotoxicity. Strain 15 exhibited high antitumour activity of R2 value 0.9802. The bio-compounds were recognized, screened and analyzed through NRPS by exact amplification.

Keywords: Endophytic actinomycetes, Streptomyces sp, Antitumour activity, HPLC and NMR analysis

Drug Discovery, 2020, 14(34), 255-264

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