Volume 14, Issue 34, July - December, 2020

Validated UV and HPLC method development for the estimation of sofosbuvirin marketed formulation

Vandana Sinha, Priyanka Chaturvedi^♦, Manish Sharma, Anurag Singh, Surendra Jain

Sagar Institute of Research and Technology, Ayodhya Bypass, Bhopal, Madhya Pradesh - 462041, India

^♦Corresponding author
E-mail address: chaturvedi2506@gmail.com

ABSTRACT

In the present research work, a successful attempt was made for Validated UV and HPLC method which was developed by experimentation based on thorough literature survey and ascertained by statistical parameters of sampling. The objective of the research work of estimation of the drug in marketed formulation. Proposed UV method was found to be linear in the range of 5-25 μg/ml Sofosbuvir with the correlation coefficient near to one (0.998). HPLC method was found to be linear in the range of 1-5 μg/ml Sofosbuvir with the correlation coefficient near to one (0.999) respectively. The isocratic mobile phase consisted of methanol: Acetonitrile in the ratio of 30:70 v/v at a flow rate of 1.0 ml min-1. A thermo C-18 column (4.6 x 250mm, 5μ particle size) was used as the stationary phase, 260.0 nm was selected as the detection wavelength for UV-vis. detector. The result obtained shows the developed methods to be Cost effective, Rapid (Short retention time), Simple, Accurate (the value of SD and % RSD less than 2), Precise and can be successfully employed in the routine analysis of those drugs in bulk drug also as in tablet dosage.

Keywords: SOFB, UN, HPLC, SD, RSD

Drug Discovery, 2020, 14(34), 328-336

PDF