In the present study, the efficiency of Polysorbate 20 (Tween 20) as additive on the removal of phenol catalyzed by horseradish peroxidase was done as an alternative to the conventional phenol removal processes. Tween 20 was found to increase the activity of horseradish peroxidase. Various studies were done by varying Tween 20 concentration, initial phenol concentration, pH and time.
Keywords: Surfactant, Tween 20, Phenol Removal, Horseradish Peroxidase, Enzymes