Vol 5, 2014
About the Cover
Indigo is a blue colored dye used from ancient times for dyeing of fabrics, now its use is also extended to food and the pharmaceutical industries. The dye is produced chemically on large scale leading to environmental pollution. Biological route for indigo production could be an alternative for sustainable indigo production. The present study focuses on the biological production of indigo from a Pandorea sp. strain, isolated from oil contaminated garage soil as an effective indigo producer in presence of indole as a substrate and silicone oil as a biphasic organic solvent. The production of pigment was confirmed using thin layer chromatography and UV visible spectrophotometer. Further for effective indigo production the following parameters like concentration of substrate-indole, pH, and temperature were optimized under single and two phase systems. When the concentration of substrate- indole, was varied from 1 to 50 mM, the maximum yield of 74.42 % was observed in single phase system at a substrate concentration of 4.686 mg/ ml whereas for two phase system the yield maximized to 91.76 % at a substrate concentration of 2.343 mg/ ml.
Bio-catalytic production of indigo using biphasic organic solvent
Vaishnavi Unde, Srikanth Mutnuri
Indigo is a blue colored dye used from ancient times for dyeing of fabrics, now its use is also extended to food and the
pharmaceutical industries. The dye is produced chemically on large scale leading to environmental pollution
Discovery Biotechnology, 2014, 5(13), 3-10
Scale-up of Antibiotic Production by Streptomyces sulfonensis
Ellaiah P, Venkateswara Rao VS, Ramakrishna SV, Venkateswarulu Y, Fadnavis N, Annapurna J
The present investigation is aimed to increase the diphenyl sulfone yield which was isolated from a new Streptomycete-
S.sulfonensis. The fermentation studies were carried out in Bioflow IV New Brunswick Scientific Edison N.I, USA 10 litre fermenter.
Discovery Biotechnology, 2014, 5(14), 13-15